plasmid plex307 dpp4 puro (Addgene inc)
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Plasmid Plex307 Dpp4 Puro, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 15 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plex307+dpp4+puro/pLEX307-DPP4-puro+(Plasmid+%23158451)/pmc12326988-40-12-22
Average 93 stars, based on 15 article reviews
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Clone Assay:Article Title: The KDM6A-KMT2D-p300 axis regulates susceptibility to diverse coronaviruses by mediating viral receptor expression Article Snippet: .. KDM6A KO clones were transduced with pLenti-hACE2-Puro (Addgene: #155295) and Article Title: DYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner. Article Snippet: .. For lentiviral transduction, Vero-E6 or Calu-3 cells were transduced at 50% confluency and selected with 5 μg/mL (Vero-E6) or 2.5 μg/mL (Calu-3) puromycin 48 hours later. hACE2 and hDPP4 overexpressing lines were generated by stable lentiviral delivery of pLV-EF1a-ACE2-puro (gift of Akiko Iwasaki) and Article Title: The KDM6A-KMT2D-p300 axis regulates susceptibility to diverse coronaviruses by mediating viral receptor expression. Article Snippet: .. KDM6A KO clones were transduced with pLenti-hACE2-Puro (Addgene: #155295) and Transduction:Article Title: The KDM6A-KMT2D-p300 axis regulates susceptibility to diverse coronaviruses by mediating viral receptor expression Article Snippet: .. KDM6A KO clones were transduced with pLenti-hACE2-Puro (Addgene: #155295) and Article Title: DYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner. Article Snippet: .. For lentiviral transduction, Vero-E6 or Calu-3 cells were transduced at 50% confluency and selected with 5 μg/mL (Vero-E6) or 2.5 μg/mL (Calu-3) puromycin 48 hours later. hACE2 and hDPP4 overexpressing lines were generated by stable lentiviral delivery of pLV-EF1a-ACE2-puro (gift of Akiko Iwasaki) and Article Title: The KDM6A-KMT2D-p300 axis regulates susceptibility to diverse coronaviruses by mediating viral receptor expression. Article Snippet: .. KDM6A KO clones were transduced with pLenti-hACE2-Puro (Addgene: #155295) and Expressing:Article Title: A trivalent protein-based pan- Betacoronavirus vaccine elicits cross-neutralizing antibodies against a panel of coronavirus pseudoviruses Article Snippet: Genscript synthesized and inserted the genes into the pcDNA3.1(+) vector (pcDNA3.1-CoV-RaTG13del19 and pcDNA3.1-CoVRsSHC014del19). .. To generate a human DPP4 (CD26) expressing target cell line (Hela-DPP4) for infection with MERS spike pseudovirions, we transduced Hela cells with a DPP4 encoding lentiviral vector, Article Title: A trivalent protein-based pan-Betacoronavirus vaccine elicits cross-neutralizing antibodies against a panel of coronavirus pseudoviruses. Article Snippet: Genscript synthesized and inserted the genes into the pcDNA3.1(+) vector (pcDNA3.1-CoV-RaTG13del19 and pcDNA3.1-CoVRsSHC014del19). .. Generation of a DPP4 expressing Hela cell line To generate a human DPP4 (CD26) expressing target cell line (Hela-DPP4) for infectionwithMERS spikepseudovirions,we transducedHela cellswith a DPP4 encoding lentiviral vector, Infection:Article Title: A trivalent protein-based pan- Betacoronavirus vaccine elicits cross-neutralizing antibodies against a panel of coronavirus pseudoviruses Article Snippet: Genscript synthesized and inserted the genes into the pcDNA3.1(+) vector (pcDNA3.1-CoV-RaTG13del19 and pcDNA3.1-CoVRsSHC014del19). .. To generate a human DPP4 (CD26) expressing target cell line (Hela-DPP4) for infection with MERS spike pseudovirions, we transduced Hela cells with a DPP4 encoding lentiviral vector, Article Title: Optimization of Cellular Transduction by the HIV-Based Pseudovirus Platform with Pan-Coronavirus Spike Proteins Article Snippet: HEK-ACE2 was obtained through BEI Resources, an NIH-supported program managed by the ATCC: Human Embryonic Kidney Cells (HEK-293T) Expressing Human Angiotensin-Converting Enzyme 2 (HEK-293T-hACE2 Cell Line, NR-52511). .. To generate a human DPP4 (CD26)-expressing target cell line (Hela-DPP4) for infection with MERS spike pseudovirions, we transduced HeLa cells with a DPP4 encoding lentiviral vector, Plasmid Preparation:Article Title: A trivalent protein-based pan- Betacoronavirus vaccine elicits cross-neutralizing antibodies against a panel of coronavirus pseudoviruses Article Snippet: Genscript synthesized and inserted the genes into the pcDNA3.1(+) vector (pcDNA3.1-CoV-RaTG13del19 and pcDNA3.1-CoVRsSHC014del19). .. To generate a human DPP4 (CD26) expressing target cell line (Hela-DPP4) for infection with MERS spike pseudovirions, we transduced Hela cells with a DPP4 encoding lentiviral vector, Article Title: Optimization of Cellular Transduction by the HIV-Based Pseudovirus Platform with Pan-Coronavirus Spike Proteins Article Snippet: HEK-ACE2 was obtained through BEI Resources, an NIH-supported program managed by the ATCC: Human Embryonic Kidney Cells (HEK-293T) Expressing Human Angiotensin-Converting Enzyme 2 (HEK-293T-hACE2 Cell Line, NR-52511). .. To generate a human DPP4 (CD26)-expressing target cell line (Hela-DPP4) for infection with MERS spike pseudovirions, we transduced HeLa cells with a DPP4 encoding lentiviral vector, Article Title: A trivalent protein-based pan-Betacoronavirus vaccine elicits cross-neutralizing antibodies against a panel of coronavirus pseudoviruses. Article Snippet: Genscript synthesized and inserted the genes into the pcDNA3.1(+) vector (pcDNA3.1-CoV-RaTG13del19 and pcDNA3.1-CoVRsSHC014del19). .. Generation of a DPP4 expressing Hela cell line To generate a human DPP4 (CD26) expressing target cell line (Hela-DPP4) for infectionwithMERS spikepseudovirions,we transducedHela cellswith a DPP4 encoding lentiviral vector, Modification:Article Title: A trivalent protein-based pan- Betacoronavirus vaccine elicits cross-neutralizing antibodies against a panel of coronavirus pseudoviruses Article Snippet: Genscript synthesized and inserted the genes into the pcDNA3.1(+) vector (pcDNA3.1-CoV-RaTG13del19 and pcDNA3.1-CoVRsSHC014del19). .. To generate a human DPP4 (CD26) expressing target cell line (Hela-DPP4) for infection with MERS spike pseudovirions, we transduced Hela cells with a DPP4 encoding lentiviral vector, Article Title: A trivalent protein-based pan-Betacoronavirus vaccine elicits cross-neutralizing antibodies against a panel of coronavirus pseudoviruses. Article Snippet: Genscript synthesized and inserted the genes into the pcDNA3.1(+) vector (pcDNA3.1-CoV-RaTG13del19 and pcDNA3.1-CoVRsSHC014del19). .. Generation of a DPP4 expressing Hela cell line To generate a human DPP4 (CD26) expressing target cell line (Hela-DPP4) for infectionwithMERS spikepseudovirions,we transducedHela cellswith a DPP4 encoding lentiviral vector, Generated:Article Title: DYRK1A promotes viral entry of highly pathogenic human coronaviruses in a kinase-independent manner. Article Snippet: .. For lentiviral transduction, Vero-E6 or Calu-3 cells were transduced at 50% confluency and selected with 5 μg/mL (Vero-E6) or 2.5 μg/mL (Calu-3) puromycin 48 hours later. hACE2 and hDPP4 overexpressing lines were generated by stable lentiviral delivery of pLV-EF1a-ACE2-puro (gift of Akiko Iwasaki) and Transfection:Article Title: Targeting DPP4-RBD interactions by sitagliptin and linagliptin delivers a potential host-directed therapy against pan-SARS-CoV-2 infections. Article Snippet: Mounting was done using fluoroshield (Sigma), and cells were visualized under a confocal laser scanning inverted microscope, Carl-Zeiss LSM980 (63×)2. .. Calu-3 cells (2.0 × 105/well) were plated onto cover glasses in 9 cm2 S. Mani et al. International Journal of Biological Macromolecules 245 (2023) 125444 well of the plates, grown overnight (to ~5 × 105 cells), and was transfected with Article Title: Targeting DPP4-RBD interactions by sitagliptin and linagliptin delivers a potential host-directed therapy against pan-SARS-CoV-2 infections Article Snippet: Mounting was done using fluoroshield (Sigma), and cells were visualized under a confocal laser scanning inverted microscope, Carl-Zeiss LSM980 (63×)2. .. Calu-3 cells (2.0 × 10 5 /well) were plated onto cover glasses in 9 cm 2 well of the plates, grown overnight (to ∼5 × 10 5 cells), and was transfected with Construct:Article Title: Targeting DPP4-RBD interactions by sitagliptin and linagliptin delivers a potential host-directed therapy against pan-SARS-CoV-2 infections. Article Snippet: Mounting was done using fluoroshield (Sigma), and cells were visualized under a confocal laser scanning inverted microscope, Carl-Zeiss LSM980 (63×)2. .. Calu-3 cells (2.0 × 105/well) were plated onto cover glasses in 9 cm2 S. Mani et al. International Journal of Biological Macromolecules 245 (2023) 125444 well of the plates, grown overnight (to ~5 × 105 cells), and was transfected with Article Title: Targeting DPP4-RBD interactions by sitagliptin and linagliptin delivers a potential host-directed therapy against pan-SARS-CoV-2 infections Article Snippet: Mounting was done using fluoroshield (Sigma), and cells were visualized under a confocal laser scanning inverted microscope, Carl-Zeiss LSM980 (63×)2. .. Calu-3 cells (2.0 × 10 5 /well) were plated onto cover glasses in 9 cm 2 well of the plates, grown overnight (to ∼5 × 10 5 cells), and was transfected with |
